Resumen

GUERRERO F, Carlos A; MARTINEZ, Constanza; SARMIENTO, Jairo  y  CARDOZO, Carmen A. OVEREXPRESESSION HEAT SHOCK COGNATE (HSC70) PROTEIN IN OSTEOBLASTS SUBMITED TO CENTRIFUGAL FORCES. rev.fac.med. [online]. 2006, vol.54, n.3, pp.169-180. ISSN 0120-0011.

Background. Osteoblasts can stimulate new bone formation by protein synthesis, such as, bone morphogenetic proteins, growth factors and collagen and non-collagenous proteins. As well, they controls reabsortion processes, because they can synthesize proteins to promote osteoclastogenic activity, when necessary. Objetive. The objetive of this work was analyze the expression of the heat shock cognate protein HSC70 in a primary culture of murine osteoblasts, after putting under a tensil force by centrifugation and determining the protein pattern by electrophoretic analysis. Materials and methods. From skulls of newborn mice ICR, cultures of cells were obtained with morphologic characteristics fibroblasts-like type, extended prolongations and cytoplasms slightly cuboidal and plump, it suggests an enriched culture of osteoblasts. Results. The technique of unidimensional electroforesis SDS-PAGE did not allow determine differences in the run pattern between osteoblasts treated with tensil force and control cells. Nevertheless, protein HSC70 overexpresion in osteoblasts was confirmed by Western blot and inmunocyto­chemistry on-are expressed. Conclusions. Electroforesis did not determine differences between control cell and cell submited to centrifugation. Stress protein HSC70 was expressed in osteoblasts submitted to centrifugación analyzed by Western's blot technology suggesting a important roll of this stres type.

Palabras clave : proteins; HSC70 heat-shock proteins; osteoblasts; electrophoresis; immunohisto­chemistry; blotting; Western.

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