Resumen

SOLANO GODOY, Jennifer Alexandra; MOLANO CARDONA, Emerson David; BERNAL BAUTISTA, Manuel Hernando  y  MURILLO ARANGO, Walter. Preliminary phospholipase, hemolytic and bactericidal activity of the venom of the snake of cascabel del Tolima. Ciencia en Desarrollo [online]. 2020, vol.11, n.1, pp.119-127.  Epub 12-Mar-2021. ISSN 0121-7488.  https://doi.org/10.19053/01217488.v11.n1.2020.9869.

In the department of Tolima, there are no studies that allow us to determine with certainty the magnitude of the ophidian accident caused by Crotalus durissus, and there is a need to generate information on the protein profile, as a way of approaching the compression of some biological activities related to the toxicity of the venom, as well as its biotechnological potential. In this work, the SDS-PAGE electrophoretic profile of the crude venom extracted from individuals collected in the municipality of Natagaima (Tolima) and the association with phospholipase, direct and indirect hemolytic and bactericidal activities on Escherichia coli, Staphylococcus aureus and Pseudomonas aeuroginosa were analysed. The crude venom presented bands of molecular weight 26.6 kDa., 17, 14.2, 6.5, 3.5 and 1.06 kDa., corresponding to other previous reports of the venom for the specie. Considerable levels of calcium-dependent-hemolytic (200 μg) and phospholipase (1.25 AU/mg. ± 0.88) activities were presented, and the bactericidal effect of the crude venom was differential on the microorganisms evaluated, showing moderate activity on Escherichia coli. The results constitute valuable data that confer an approach towards the knowledge of the toxic potential of the venom of Crotalus durissus (rattlesnake) from the Natagaima-Tolima area, as well as the bactericidal capacity and possible future applications in research fields related to the search for new antimicrobial agents.

Palabras clave : Crotalus durissus; Bactericidal activity; Hemolysis; Phospholipases; Rattlesnake; Toxinology; Venom.

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