Resumen

CARRERA PAEZ, Laura Camila et al. Comparison of cell culture HeLa and HEp-2: Studies prospects Chlamydia trachomatis. Nova [online]. 2015, vol.13, n.23, pp.17-29. ISSN 1794-2470.

Objective. The goal of this study was to standardize the cultivation of HeLa cells under different conditions, to be used in Chlamydia trachomatis serovar L2 infection's protocols. Materials and Methods. This study was conducted in four phases that are: 1.Cell Viabilidad by trypan blue technique and his subsequent remark, 2. Standardization HeLa cell culture, 3. Giemsa, 4. Cultivation of Hep-2 cells. Results. As a result of standardization it is determined that the HeLa cell line should be cultured in DMEM, 0.1% L-glutamine, 10% FBS.It was determined that the Giemsa perform better over time of 40 minutes that a clear definition of nucleus and cytoplasm is evident. Comparing against both cell lines HeLa was obtained that the line from day growing well and quickly reaches the expected confluence, the opposed line HEp-2 has a slower growth but achieving the desired confluence the last day.

Palabras clave : Chlamydia trachomatis; HeLa cells; HEp-2 cells; Cell culture; Cell infection.

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