Servicios Personalizados
Revista
Articulo
Indicadores
- Citado por SciELO
- Accesos
Links relacionados
- Citado por Google
- Similares en SciELO
- Similares en Google
Compartir
Revista Colombiana de Entomología
versión impresa ISSN 0120-0488versión On-line ISSN 2665-4385
Resumen
GIRALDO H., PAULA ANDREA; URIBE S., SANDRA INÉS y LOPEZ R., ANDRÉS. Analysis of mitochondrial DNA sequences (Cytb and ND1) in Lucilia eximia (Diptera: Calliphoridae) . Rev. Colomb. Entomol. [online]. 2011, vol.37, n.2, pp.273-278. ISSN 0120-0488.
Lucilia eximia (Diptera: Calliphoridae) is a species of forensic importance for its association with decomposing bodies in Antioquia and other regions of Colombia. The implementation of methods to allow the rapid and accurate identification of this species is a matter important in forensic entomology. Currently, the mitochondrial DNA sequences are considered to complement classical taxonomy and are used in the characterization of morphologically similar or indistinguishable species as in the case of forensic importance fies. We evaluated the variability of a mitochondrial region (Cytb -RNAt-Ser-ND1) of 480 bp that was amplifed by PCR, from L. eximia larvae on third stage from Medellin and two other localities. The sequence differences evaluated as nucleotidic differences and genetic distances permitted the separation of individuals from the species of interest when compared with morphologically similar and related species. Furthermore, using a N.J analysis (K2p) the haplotype groupings were related to the geographical origin of individuals from L. eximia.
Palabras clave : Forensic Entomology; Citochrome b; Molecular markers.