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Revista Colombiana de Biotecnología
versão impressa ISSN 0123-3475
Resumo
ALVAREZ OJEDA, María Genoveva; PECINA QUINTERO, Víctor; ACOSTA DIAZ, Efraín e ALMEYDA LEON, Isidro Humberto. Molecular evaluation of the wild avocado (Persea americana Mill) in Nuevo León, México. Rev. colomb. biotecnol [online]. 2018, vol.20, n.2, pp.38-46. ISSN 0123-3475. https://doi.org/10.15446/rev.colomb.biote.v20n2.69551.
México is the center of origin of the avocado (Persea americana Mill), most of the recognized members of the genus Persea occur primarily from the central part of México to Central América. In this study, the molecular evaluation of germplasm of wild avocado from the State of Nuevo León, México, was performed using Random Amplified Polymorphic DNA (RAPD's) technique. A total of 27 wild avocado materials were collected in the southern region and 16 in the northern region of Nuevo León. The level of gen et-ic diversity detected was 84%, which is considered high. Specific fragments or only RAPD's type, present in a single individual, were observed, this type of fragments are of particular interest since they can be linked to a particular genotype and serve in the diagnosis to differentiate a genotype or a specific region of the genome. The above is of particular interest for the creole avocado of the State of Nuevo León, whose problem for marketing is the short shelf life that presents, therefore, finding great genetic variation as detected in this work increases the possibility of generating new materials whose shelf life is longer, enhancing its commercial value.
Palavras-chave : Genotypes; RAPD's; Genetic Diversity; Polymorphism.