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Revista de la Universidad Industrial de Santander. Salud
versão impressa ISSN 0121-0807
Resumo
SIERRA BALCARCEL, Raúl et al. Standardization of ELISA for the diagnosis of bovine, ovine and human fasciolosis. Rev. Univ. Ind. Santander. Salud [online]. 2017, vol.49, n.4, pp.549-556. ISSN 0121-0807. https://doi.org/10.18273/revsal.v49n4-2017004.
Introduction:
Fascioliasis represents a serious problem for livestock production and human health in the world. In Colombia, it mainly affects bovine and ovine cattle, with few cases reported in humans.
Objective:
To standardize an Enzyme-Linked ImmunoSorbent test (ELISA) in bovine, ovine, and human individuals as a screening tool of fascioliasis.
Methods:
50 bovine, 50 ovine and 39 human samples with fascioliasis confirmed by serology and stool test were used. Sensitivity, specificity and concordance were calculated.
Results:
Sensitivity of the ELISA in humans, bovine and ovine cattle was 100 % and specificity was 97%, 85.2% and 96.2%, respectively. Cohen´s Kappa coefficient was >0.8 in all groups.
Conclusions:
Standardized ELISA test for bovine, ovine and human fascioliasis exhibited an excellent sensitivity and good specificity. It is proposed to carry out a large-scale validation for its ulterior use as a screening tool for the diagnosis of this parasitic infection.
Palavras-chave : Enzyme-Linked Immunosorbent Assay; diagnosis; fascioliasis; Fasciola hepatica; standardization; humans; sheep; cattle.