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Ciencia y Tecnología Agropecuaria
versão impressa ISSN 0122-8706
Resumo
ERASO INSUASTY, Christian; ACOSTA RODRIGUEZ, Jairo; SALAZAR GONZALEZ, Claudia e BETANCOURTH GARCIA, Carlos. Evaluation of Trichoderma spp. strains for control yellowing pea caused by Fusarium oxysporum. Corpoica cienc. tecnol. agropecu. [online]. 2014, vol.15, n.2, pp.237-249. ISSN 0122-8706.
The yellowing of pea caused by the fungus Fusarium oxysporum f. sp. pisi is considered the most damaging disease of this crop. This study took place at the plant health laboratory and greenhouse of the Universidad de Nariño, and the experimental stage was conducted at the Granja experimental Botana. Its purpose was to evaluate the antagonistic ability of the fungi Trichoderma spp. to F. oxysporum. Isolation of F. oxysporumwas made from diseased tissue; Trichoderma strains were obtained from the rhizosphere of healthy plants (collected in the towns of Potosi, Córdoba, Gualmatán, Ipiales and Puerres in the state of Nariño, Colombia), and a commercial strain from laboratory Perkins Ltda. In laboratory, unrestrictedly randomized design with 21 treatments (strains) was used. Mycelial growth and inhibition zone were evaluated in dual plantings, which served as selection criteria for greenhouse test where plant height, root length, root dry matter and percentage of incidence were evaluated. In the field, a randomized block design was used to evaluate yield components, plant height and root length with the best strains. In the laboratory, C2 (Córdoba 2), C7 (Gualmatán 3), C14 (Puerres 2), C20 (Potosi 4) and C21 (Perkins Lab.) showed antagonistic activity in the greenhouse, C7, C14 and C21 were the best; in field, significant differences between C14 and C21, compared to C7 and the control, were obtained. Strains C14 and C21 have consistent antagonistic capacity and can be used to control F. oxysporumin pea.
Palavras-chave : biological control; soil pathogen; antagonism; Pisum sativum L..