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Revista de Salud Pública
versão impressa ISSN 0124-0064
Resumo
AGUDELO-SUAREZ, Angela Natalia et al. Identification and genomic sequencing of SARS-CoV-2 in an African lioness (Panthera leo), under professional technical care in Colombia. Rev. salud pública [online]. 2022, vol.24, n.2, pp.1-. Epub 15-Jun-2023. ISSN 0124-0064. https://doi.org/10.15446/rsap.v24n2.104984.
Objective
To identify and characterize the SARS-CoV-2 virus in an elderly African lioness (Panthera leo) that presented signs related to atypical respiratory disease for several months.
Methods
Nasal swab samples were taken 23 days after infection. have reported initial nasal discharge.
Results
The SARS-Cov2 virus was detected by RT-qPCR and the complete genome was subsequently characterized by Illumina sequencing. The results found in the blood samples did not show obvious changes that could be related to the virus or to the signs described from the beginning of the case. For genomic sequencing the analyzes showed a comparative multiple alignment between the three genomes (sample Leona, FIP or NC_045512 (Wu han)) by means of Mauve, focusing on the Spike, E and M genes (Supplementary file, part B); 5 very similar segments between Leona and NC_045512 (Wuhan) was identified.
Conclusions
It is necessary to carry out more research to standardize the diagnosis of this pathology in animals and guarantee access to it. Also, genomic studies in these species. Additionally, it was evidenced with the literature review that there are many knowledge gaps in the zoonotic implication of the Pandemic and in the knowledge of this virus in domestic and wild animals, which represents an important challenge for research from now on.
Palavras-chave : RT-qPC; coronavirus infections; lions; wild animals; Illumina sequence (source: MeSH, NLM).