Resumo

CORRALES-ALVAREZ, Juan D. et al. STUDY OF HINFI POLYMORPHISMS OF THE PIT-1 GENE AND THEIR ASSOCIATIONS WITH TYPE TRAITS, MILK YIELD AND DAYS OPEN IN HOLSTEIN COWS FROM ANTIOQUIA, COLOMBIA. Actu Biol [online]. 2010, vol.32, n.93, pp.139-145. ISSN 0304-3584.

The aim of this study was to determine allele and phenotypic frequencies for single nucleotide polymorphism (SNP) of exon VI in the Pit-1 gene and their associations with type traits, milk yield, and days open. A total of 390 Holstein cows from Antioquia (Colombia) were genotyped for the Pit-1 Hinfi polymorphism by PCR-RFLP. We found AA, AB, and BB genotypes with a frequency of 0.03, 0.43, and 0.53, respectively. The A allele frequency was 0.35 and its presence in the genotype was associated with higher milk production, udder depth and body depth; the absence of the A allele in the genotype was associated with fewer days open. This study indicates that it is possible to develop breeding programs using the Pit-1 gene in Holstein cattle in the department of Antioquia.

Palavras-chave : animal breeding; Colombia; Holstein cows; molecular marker; Pit-1.

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