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Biosalud
versão impressa ISSN 1657-9550
Resumo
CABELLO-VILCHEZ, Alfonso Martín e NUNEZ-ATO, Ronald G.. ISOLATION AND MOLECULAR CHARACTERIZATION OF NONTUBERCULOUS MYCOBACTERIA IN THE WATER DISTRIBUTION SYSTEM IN A HOSPITAL OF LIMA (PERÚ). Biosalud [online]. 2018, vol.17, n.2, pp.7-24. ISSN 1657-9550. https://doi.org/10.17151/biosa.2018.17.2.1.
Introduction:
Mycobacterial infections can cause skin and lung lesions. Mycobacteria can be found in intra-hospital water sources which can reach immuno-suppressed patients and cause severe disease.
Objective:
To isolate and characterize non-tuberculous mycobacteria in the water distribution system in a hospital in Lima.
Materials and Methods:
Mycobacteria were isolated in conventional culture egg-based media and biochemical tests and molecular tools such as polymerase chain reaction (PCR) and sequencing were used for their identification. The GenBank was used for sequencing analysis and MEGA 6 was used for the phylogenetic tree.
Results:
Isolation of acid-fast bacilli was possible in samples of cold drinking water, although not in the hospital hot water. Several species of non-tuberculous mycobacteria were isolated and identified in the water samples being a 100% of rapid growth such as Mycobacterium canariasense, M. frederiksbergense, Mycobacterium fortuitum, Mycobacterium chelonae, and M. mucogenicum.
Conclusions:
This is the first time that mycobacteria are isolated in drinking water at a hospital in Perú. It is concluded that the existence of mycobacteria in hospital water could be a potential source of nosocomial outbreaks and should be analyzed periodically.
Palavras-chave : atypical mycobacteria; soil and water Mycobacterium; non-tuberculous mycobacteria.