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Biotecnología en el Sector Agropecuario y Agroindustrial
versão impressa ISSN 1692-3561
Resumo
PATINO TORRES, CARLOS e SANCHEZ DE PRAGER, MARINA. ISOLATION AND IDENTIFICATION OF PHOSPHATE-SOLUBILIZING BACTERIA INHABITING THE RIZHOSPHERE OF PEACH PALM (B. gassipaes Kunth). Rev.Bio.Agro [online]. 2012, vol.10, n.2, pp.177-187. ISSN 1692-3561.
The chontaduro or pejiyabe (Bactris gasipaes Kunth), is a species native of rainforest of Colombian's Pacific Coast, adapted to soils with high acidity and depleted in nutrients, especially phosphorus.The phosphate solubilizing microorganisms, including bacteria (BSP), improve the availability of this nutrient, therefore, the need to isolate, recognize and identify those in the rhizosphere, as one of the mechanisms of adaptation of this species. Once isolated and purified in medium free of sources of soluble phosphorus, bacterial populations were identified using sequence analysis of 16S rDNA and molecular genotyping by BOX-PCR, stating that in the study site, the dominant populations were Burkholderia ambifaria and B. sp. 383, followed by Pseudomonas putida, species recognized globally for its solubilizing activity, and as plant growth promoting rhizobacteria (PGPR).
Palavras-chave : Phosphate solubilization; Soil phosphorus; Rhizosphere.