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versão impressa ISSN 1794-2470
Resumo
CHAPARRO PEDRAZA, Aura Patricia e CAMPUZANO F., Silvia E.. Isolation identification and evaluation of the antimicrobial activity for metabolites produced by Mucor circinelloides. Nova [online]. 2018, vol.16, n.29, pp.63-70. ISSN 1794-2470.
Objective.
To identify and evaluate the antimicrobial capacity of the metabolites isolated from the fermentation process in a strain of Mucor circinelloides.
Method.
For this study it was taken the 321 SPG native strain of Mucor circinelloides provided by GIBUJ. The kinetics growth was made, observing the ending of the idiofase at hour 264, hour taken for the conclusion of fermentation. Mycelium was separated from the broth and then purified with thin layer chromatography and silica gel column, eluted with different relations of solvents.
Results.
The results given by the technique of coupled gases with GC-MS mass confirm the importance of Mucor circinelloides in the production of unsaturated fatty acids. A sterol was obtained from the mycelium, M.cB3 compound. The fraction CHCl3 in biomass showed inhibitory activity for Gram-positive microorganisms.
Palavras-chave : Mucor circinelloides; unsaturated fatty acids; antimicrobial activity; secondary metabolites.