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Revista Colombiana de Ciencias Pecuarias
versão impressa ISSN 0120-0690versão On-line ISSN 2256-2958
Resumo
TRUJILLO BRAVO, Esperanza e VALDERRAMA LLANOS, Yasbleydy. Genotipificación de la región 3´UTR del gen Nramp1, en ganado Holstein y en criollo Hartón del Valle. Rev Colom Cienc Pecua [online]. 2006, vol.19, n.4, pp.401-406. ISSN 0120-0690.
NRAMP1: Genetic characterization at the 3´UTR region in Holstein and creole Hartón del Valle cattle Summary In studies of natural resistance or susceptibility to different intracellular pathogens the Nramp1 gene has been described as controlling this characteristic in mouse and in the humans. In this project we genotyped 151 Harton of the Valle bovine individuals and 128 Holstein, for a microsatellite of the 3’ untranslated region of Nramp1 gene, in position 1745-1955 and found that the most frequency size of microsatellite were 207 and 211 bp. The sequencing results indicate that there is polymorphim in the number of GT repetitions and (GT)12 and (GT)11 were present as reported previously, as well as (GT)14 y (GT)10.
Palavras-chave : GTs; pathogens; repeats; sequencing; susceptibility; 3UTR.