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Biomédica
versão impressa ISSN 0120-4157versão On-line ISSN 2590-7379
Resumo
ARTETA, Ariel A.; ARIAS, Luis F. e CADAVID, Claudia E.. Ziehl-Neelsen stain in the pathology laboaratory: Performance and diagnostic aid for mycobacteria in bronchoalveolar lavage. Biomed. [online]. 2022, vol.42, n.3, pp.460-469. Epub 02-Set-2022. ISSN 0120-4157. https://doi.org/10.7705/biomedica.6347.
Introduction:
With more than 100 years of use, the Ziehl-Neelsen stain is still currently used worldwide.
Objective:
To compare the performance of diagnostic tests used to determine mycobacteria in the clinic and pathology laboratory in bronchoalveolar lavage samples.
Materials and methods:
We retrospectively reviewed 737 bronchoalveolar lavage samples from 2019 to 2020 in the San Vicente Fundación hospital (Medellín, Colombia) comparing the performance of three tests done in parallel: mycobacteria and resistance PCR, culture, and Ziehl-Neelsen stain.
Results:
In total, 93/737 patients were classifed as sick due to a positive result in any of the three tests. The culture, PCR, and Ziehl-Neelsen stain had a sensibility of 0.80, 0.76 y 0.51, respectively. However, only 5/75 (6.5%) of the positive cultures had results within the frst four weeks and the rest in eight weeks. The PCR test combined with the Ziehl-Neelsen stain improved the sensibility of the PCR test alone from 0.76 a 0.88, a change that was statistically signifcant (p = 0.022).
Conclusion:
At least in bronchoalveolar lavage samples, culture is still the test with better sensibility. The use in parallel of the PCR test and the Ziehl-Neelsen stain improved in a statistically signifcant manner the performance of the PCR test alone, regardless of the higher turnaround time of the Ziehl-Neelsen stain.
Palavras-chave : Tuberculosis/diagnosis; bronchoalveolar lavage; sensitivity and specifcity; Colombia.