Abstract

MAESTRE-SERRANO, Ronald; GUEVARA-ROZO, Edilma; COLMENARES-DE ESCAMILLA, Irma  and  PACHON-MUNOZ, Ernesto. L-lactate: NAD⁺ Oxide-reductase (LDH; EC. 1.1.1.27) isoenzymes expression during embryonary development of siamese fighting fish Betta splendens (REGAN, 1909). Univ. Sci. [online]. 2008, vol.13, n.1, pp.11-20. ISSN 0122-7483.

Expression of the L-lactate: NAD+ oxide-reductase (LDH; EC. 1.1.1.27) isoenzymes, was reported during embryonic development of Betta splendens using electrophoretic methods. Two hundred eggs were taken in each state of embryonic development (fertilized oocite, clivajes, blástulas, gástrula and neurula), from four sexually mature couples of fish Betta splendens. Oocites without fertilizing from two females of the same species were collected. Each sample of 200 embryos and 200 eggs without fertilizing, were homogenized and centrifugated during 15 minutes to 10.000 rpm. Electrophoresis was run in agarosa gel. All the stages of embryonic development studied were characterized by expression of isoenzymes LDH- 3 and LDH-4. Gastrula and neurula stadiums also expressed isoenzima LDH-2.

Keywords : Betta splendens; expression; isoenzymes; lactate dehydrogenase.