Susceptibility of Plutella xylostella (Lepidoptera: Plutellidae; Linnaeus 1758) to Beauveria bassiana Bb9205, Metarhizium anisopliae Ma9236 and Heterorhabditis bacteriophora HNI0100

Susceptibilidad de Plutella xylostella (Lepidoptera: Plutellidae; Linnaeus 1758) a Beauveria bassiana Bb9205, Metarhizium anisopliae Ma9236 y Heterorhabditis bacteriophora HNI0100

Susceptibilidade de Plutella xylostella (Lepidoptera: Plutellidae; Linnaeus 1758) a Beauveria bassiana Bb9205, Metarhizium anisopliae Ma9236 e Heterorhabditis bacteriophora HNI0100

J.P. Correa-Cuadros¹, M.X. Rodríguez-Bocanegra², A. Sáenz-Aponte¹

Edited by Alberto Acosta

¹Laboratorio de Control Biológico. Grupo de Biología de Plantas y sistemas productivos. Departamento de Biología. Facultad de Ciencias. Pontificia Universidad Javeriana. Bogotá, D.C., Colombia.
²Unidad de Investigaciones Agropecuarias, UNIDIA. Departamento de Microbiología. Facultad de Ciencias. Pontificia Universidad Javeriana. Bogotá, D.C., Colombia.

Funding: Own resources of Laboratorio de Control Biológico and Unidad de Investigaciones Agropecuarias, Pontificia Universidad Javeriana.
Electronic supplementary material: N/A

Received: 03-02-2014 Accepted: 25-03-2014 Published on line: 16-06-2014

Para citar este artículo / To cite this article

Correa-Cuadros JP, Rodríguez-Bocanegra MX, Sáenz-Aponte A (2014) Susceptibility of Plutella xylostella (Lepidoptera: Plutellidae; Linnaeus 1758) to Beauveria bassiana Bb9205, Metarhizium anisopliae Ma9236 and Heterorhabditis bacteriophora HNI0100. Universitas Scientiarum 19(2): 277-285 doi: 10.11144/Javeriana.SC19-2.spxl

Abstract

The diamondback moth (Plutella xylostella) is a major pest of broccoli worldwide. It mainly causes leaf defoliation and generates annual losses of 80%. In this study we evaluated the susceptibility of P. xylostella to entomopathogens Heterorhabditis bacteriophora HNI0100, Beauveria bassiana Bb9205 and Metarhizium anisopliae Ma9236. The methodology was based on the inoculation of third instar larvae of P. xylostella with 5x10\ 1x10², 3x10², 6x10² and 1,2x10³ IJs/cm² of H. bacteriophora HNI0100 and evaluated them after 24, 48 and 72 h and 1x10⁴, 1x10⁵, 1x10⁶, 1x10⁷ and 1x10⁸ con/cm² of B. bassiana Bb9205 and M. anisopliae Ma9236, which were evaluated during two weeks. At a dose of 1,2 x10³ JIs/cm², P. xylostella had a susceptibility to H. bacteriophora HNI0100 of 91,66%. Similarly, B. bassiana Bb9205 and M. anisopiae Ma9236 had a mortality of 95,33 and 99,67% at 1x10⁵ con/cm². The results suggest that the use of strains of entomopathogenic nematodes and fungi is an innovative alternative for the control of P. xylostella. However, studies on the interaction of nematodes and fungi and Plutella xylostella are necessary.

Keywords: Broccoli; biological control; diamondback moth; entomopathogenic fungi; entomopathogenic nematodes.

Resumen

La palomilla dorso de diamante (Plutella xylostella) es una de las principales plagas del cultivo de brócoli (Brassica oleracea) en el mundo. El principal daño es la defoliación de las hojas, generando pérdidas anuales del 80%. El objetivo de este estudio fue evaluar la susceptibilidad de P. xylostella a los entomopatógenos Heterorhabditis bacteriophora HNI0100, Beauveria bassiana Bb9205 y Metarhizium anisopliae Ma9236. La metodología se basó en la inoculación de 5x10¹, 1x10², 3x10², 6x10² y 1,2x10³ JIs/cm² en larvas de tercer instar de P. xylostella evaluada a las 24, 48 y 72 h y 1x10⁴, 1x10⁵, 1x10⁶, 1x10⁷ y 1x10⁸ con/cm² de B. bassiana y M. anisopliae evaluadas durante dos semanas. Los resultados mostraron que P. xylostella fue susceptible a H. bacteriophora HNI0100 con una tasa de mortalidad del 91,66% a dosis de 1,2x10³ JIs/cm². Así mismo, B. bassiana Bb9205 y M. anisopliae Ma9236 generaron 95,33 y 99,67% de mortalidad con la dosis de 1x10⁵ con/cm². El uso de nematodos y hongos entomopatógenos es una alternativa innovadora para el control de P. xylostella, sin embargo, se requiere estudiar su interacción para el control de este insecto plaga.

Palabras clave: Brócoli; control biológico; hongos entomopatógenos; nematodos entomopatógenos; palomilla dorso de diamante.

Resumo

A mariposa dorso de diamante Plutella xxylostella é uma das principais pragas do cultivo dos brócolos (Brassica oleraceà) no mundo. O principal dano é o desfolhamento das folhas, gerando perdas anuais de 80%. O objetivo deste estudo foi avaliar a suceptibilidade da P. xylostella aos entomopatógenos Heterorhabditis bacteriophora HNI0100, Beauveria bassiana Bb9205 e Metarhizium anisopliae Ma9236. A metodologia baseou-se na inoculação de 5x10¹, 1x10², 3x10², 6x10² y 1,2x10³ JIs/cm² em larvas do terceiro instar de P. xylostella avaliada às 24, 48 e 72 h e 1x10⁴, 1x10⁵, 1x10⁶, 1x10⁷ e 1x10⁸ con/cm² de B. bassiana e M. anisopliae avaliadas durante 2 semanas. Os resultados mostraram que P. xylostella foi susceptível a H. bacteriophora HNI0100 com uma taxa de mortalidade de 91,66% com a dose de 1,2x10³ JIs/ cm². Desta forma, B. bassiana Bb9205 e M. anisopliae Ma9236 geraram 95,33 é 99,67% de mortalidade com a dose de 1x10⁵ con/cm². O uso de estirpes colombianas de nematóides e fungos entomopatógenos é uma alternativa inovadora para o controlo de P. xylostella. Ainda se requer estudar a interação entre fungos e nematóides em Plutella xylostella.

Palavras-chave: Brócolos; controlo biológico; fungos entomopatógenos; nematóides entomopatógenos; mariposa dorso de diamante.

Introduction

One of the main pests affecting broccoli (Brassica oleracea) is the diamondback moth, Plutella xylostella (Linnaeus 1758; Lepidoptera: Plutellidae; Bertolaccini et al. 2010, Sáenz 2012). Worldwide, this pest generates losses of over 80% (U.S. $4 to U.S. $5 billion) in annual crop production (Table 1) (Verkerk & Wright 1996, Sarfraz et al. 2005, Zalucki et al. 2012).

The diamondback moth has a four-stage life cycle (egg, larva, pupa and adult) of 15 to 40 days depending on regional weather conditions; the entire life cycle takes place on the leaves abaxial surface of the host plant (Sarfraz et al. 2005). The egg-stage lasts 3.2 days at 20 °C. During the 15 days of its larval instars (four instars), the pest causes the greatest damage to broccoli crops (Somvanshi & Ganguly 2007). White spots on the leaves evidence surperficial mines that alter the photosynthesis process, and cause a decrease in the size and quality of the product intended for consumption (Franco 2001, Schroer et al. 2005, Chavez & Hurtado 2010). Eighteen days before the adult hatches, the pupa is covered with a silk thread on the abaxial surface of the leaf (Talekar & Shelton 1993, Chavez & Hurtado 2010). Adults are nocturnal; males emerge seeking a partner for copulation and are found on the leaves during the day. The average adult female lays 160 to 360 eggs (Furlong et al. 2013).

This insect pest is controlled through the continued use of agrochemicals. A 99-day crop can be treated three to five times with insecticides such as organophosphates, pyrethroids and carbamates (Sarfraz et al. 2005). The indiscriminate use of these products by farmers has led to excessive application of the same and an increase in resistance issues with this lepidopteran pest (Monzón 2001, Sáenz 2012). For this reason, other alternatives of control have been evaluated such as physical barriers, as light and resistant covers to prevent the pest from accessing the plants (Chavez & Hurtado 2010) or biological control using parasitoids such as Diadegma insulare or Cotesia plutellae or entomopathogenic fungi and nematodes, which have shown significant advance in managing the diamondback moth (Sarfraz et al. 2005, Schroer et al. 2005, Bertolaccini et al. 2010). With a 85% mortality, the entomopathogenic nematode, Heterorhabditis bacteriophora (Poinar 1976) (Rhabditida: Heterorhabditidae) is one of the species with the greatest potential to control P. xylostella (Shinde & Singh 2000, Somvanshi & Ganguly 2007). This nematode species is a cruise-type forager that can enter through the anus, spiracles or cuticle (Sánchez 2002). It is hermaphrodite during the first generation of its life cycle and has a tooth that allows it to perforate any insect cuticle, which gives it a competitive advantage over other biocontrol agents (Sáenz & Lopez 2010).

Entomopathogenic fungi, Beauveria bassiana (Balsamo-Crivelli) Vuillemin (Cordycipitaceae) and Metarhizium anisopliae (Metschnikoff) Sorokin (Clavicipitaceae) have been widely used to control P. xylostella (Loc & Chi 2007), producing over 50% mortality (Furlong & Pell 2001, Pucheta et al. 2006, Hui Wu et al. 2010). Although they do not enter through the mouth or anus, they have the ability to adhere to the insect cuticle and penetrate it using cuticle-degrading enzymes to carry out an effective colonization (Loc & Chi 2007).

The success of using entomopathogenic nematodes and fungi in biological control strategies depends on the strains of natural enemies and their relationship with the pest (Sarfraz et al. 2005). The populations of M. anisopliae, B. bassiana and H. bacteriophora differ genetically and biologically depending on the region in which they are isolated; therefore, it is necessary to assess their virulence and pathogenicity to the pest to be controlled (Furlong et al. 2013).

The objective of this study was to assess the susceptibility of P. xylostella to H. bacteriophora HNI0100, B. bassiana Bb9205 and M. anisopliae Ma9236 for future studies on the interaction of fungi and nematodes to control diamondback moth.

Materials and methods

Entomopathogens: H. bacteriophora HNI0100, M. anisopliae Ma9236 and B. bassiana Bb9205. After screening different strains of Steinernematidae and Heterorhabditidae, entomopathogenic nematode H. bacteriophora HNI0100 was used for the laboratory tests (Delgado-Ochica & Sáenz 2012). The Biological Control Laboratory at Pontificia Universidad Javeriana provided the infective juveniles, which were multiplied in vivo by infecting last instar larvae of Galleria mellonella (Linnaeus 1756) (Lepidoptera: Pyralidae) following the methodology of Kaya & Stock (1997). The entomopathogenic fungi B. bassiana Bb9205 and M. anisopliae Ma9236 were obtained from the Laboratorio de Control de Calidad de Bioinsumos Agrícolas -Control de Bioinsumos Disciplina de Entomolog'a, Cenicafé, Chinchiná - Caldas - Colombia. The fungi were activated by infecting P. xylostella. For the optimal growth and production of conidia, the reactivated strains were grown in PDA (potato dextrose agar) and oatmeal agar for 15 days at 25 °C.

Entomopathogenic Evaluation: The susceptibility of P. xylostella to H. bacteriophora HNI0100, B. bassiana Bb9205 and M. anisopliae Ma9236 was evaluated by transferring one larva of third instar to 2 oz plastic containers, each one containing 5 g of broccoli leaf and 60 g of sterile river sand (Table 2). The infective juveniles (IJs) were suspended in distilled water with Tween 80 (0.1 % v/v) and inoculated over the sand and broccoli leaf. Larvae mortality by entomopathogenic nematodes was evaluated during 24, 48 and 72 hours. Subsequently, once dead, the larvae were transferred to White traps to recover infective juveniles. Fungal conidia were suspended in saline solution with Tween 80 (0.1 % v/v) and inoculated over the sand and broccoli leaf. Larvae mortality by entomopathogenic fungi was evaluated during two weeks.

Statistical Analysis: P. xylostella mortality percentages were tested for variance homogeneity (Levene) and normality (Kolmogorov-Smirnov); the assumptions for parametric data were met, demonstrating that treatment variances were the same and their errors had a normal distribution. A univariate ANOVA with a factorial arrangement was used to determine whether dose and time means were the same or different in relation to the mortality percentage of each test (entomopathogenic nematodes and fungi) and to determine the interaction of both factors. Subsequently, multiple comparisons using Tukey and Scheffe tests were performed, employing a 95% probability, to identify the dose with the highest mortality of P. xylostella larvae, as well as the time during which the highest mortality occurred. The tests were performed using Statistix 10 software.

Results

Third instar larvae of P. xylostella were susceptible to H. bacteriophora HNI0100 with significant differences between doses (F=5002.48, p=0.0000) and time (F=2614.75, p=0.0000) (Figure 1a). The post-hoc tests indicated that the dose with the highest mortality was 1,2x10³ IJs/cm² with a percentage of 91,66%. Similarly, the highest larvae mortality occurred at 48 and 72 hours post-inoculation. This experiment replicated the interaction between dose and time (F=205.71, p=0.0000) mentioned by Goettel et al. (1993). Larvae cavaders displayed a yellow-ocher coloration, which is an indicative of a IJs infection (Figure 2a). Additionally, the IJs migrated from cadavers (Figure 2b).

Third instar larvae were susceptible to M. anisopliae Ma9236 (Figure 2c) and B. bassiana Bb9205 (Figure 2d and 2e), presenting a characteristic fungal symptomatology. Significant differences between doses (F=222.06, p=0.0000) and time (F=274.57, p=0.0000) were found for B. bassiana Bb9205 (Figure 1b), M. anisopliae Ma9236 behaved equally with dose (F=1529.34, p=0.0000) and time (F=11060.65, p=0.0000) (Figure 1c).

The highest mortality produced by B. bassiana Bb9205 (95,33%) was at a dose of 1x10⁵ con/cm² 15 days after inoculation, and by M. anisopliae Ma9236 (99,67%) was at a dose of 1x10⁵ con/cm² 15 days after inoculation. The interaction between dose and time for B. bassiana (F=32.15, p=0.0000) and M. anisopliae (F=171.94, p=0.0000) suggests a trend of increasing mortality over time.

Discussion

Third instar larvae of P. xylostella were susceptible to H. bacteriophora HNI0100. The highest mortality, a percentage of 91,66%, occurred with the dose of 1,2x10³ IJs/cm² at 72 hours post-inoculation. A study by Sáenz (2012) obtained similar data, the mortality rate in this study was of 95%. Ratansinghe & Hauge (1995) and Nyasani et al. (2008 a and b) also obtained mortality rates of 85 to 95% with H. indica.. The results obtained by Salem et al. (2007) demonstrated that the percentage of mortality increased with time, establishing that the mortality generated in the first 48 hours is due to a mutualistic relationship with Photorhabdus luminescens (Poinar 1976), which gives the nematode a competitive advantage allowing it to kill its host quickly (Mason & Wright 2000, Sáenz 2012). According to Mason & Wright (1997) and Baur et al. (1995), the yellow-ocher coloration displayed by P. xylostella after infection by H. bacteriophora HNI0100 is not the characteristic symptomatology for Heterorhabditidae, which usually display a reddish hue. This divergence is attributed to the insect's original color (green), the concentration of bacterial cells of P. luminescens in each infective juvenile that enters the larva (30-200 IJs), the concentration of bacteria in the insect's hemolymph and the refraction of light on the cuticle (Silva et al. 2002, Waterfield et al. 2009). The larvae had a symptomatology characteristic of infection by nematodes, displaying flaccidity and little mobility, as reported by Nyasani et al. (2008).

Third instar larvae were susceptible to B. bassiana Bb9205 and M. anisopliae Ma9236. Both fungi had the highest mortality at a dose of 1x10⁵ con/cm² on day 15 after inoculation; this is comparable to results obtained by Franco (2001), who establish that the percentage of mortality increases with time, and is corroborated by studies by Loc & Chi (2007), Thuy (2001) Butt & Goettel (2000), that conclude that fungal pathogenicity is characterized by a gradual increase in mortality with extended exposure time. According to Quesada-Moraga & Vey (2004), Hui Wu et al. (2010) and Anaisie et al. (2011), concentrations of 1x10⁵ con/cm² are low to cause a mortality percentage exceeding 60% in P. xyostella; these authors recommend the use of concentrations in excess of 1x10⁹ con/cm² to produce a larval mortality of 80%. This study demonstrates that a high mortality can be obtained at low-doses, depending on the species and strain of the entomopathogenic fungus (Roy & Pell 2000, Furlong & Pell 2001, Sun et al. 2002, Furlong 2004). Sarfraz et al. (2005) indicate that the success of biological control strategies depends on the accurate identification of natural enemies and association with the strain; misidentifications can prompt the failure

of the pest management program. Ibrahim & Low (1993) and Shelton et al. (1998) state that strains of entomopathogenic fungi produce different responses in mortality of individuals because their virulence and pathogenicity are not the same. By conducting tests on P. xylostella with six isolates of B. bassiana and two of M. anisopliae, Godonou et al. (2009) found that the strain with the highest mortality (94%) was Bba5653, establishing that the percentage and death rate of the pest can be variable. A study by Vandenberg et al. (1998) determined that the survival times for P xylostella larvae, inoculated at different doses, were variable for two strains of B. bassiana, pathogenicity decreased with an increased dose, which agrees with our results. They also established that survival times and mortality vary between the two isolates, this is related to strains and their virulence.

The relationship between doses, mortality, biological controllers and pest generally responds to a positive correlation between the dose and the pathogen ability to kill the pest (Pena et al. 1991, Ferron 1981, Butt & Goettel 2000). In this study, the nematodes dose with the highest mortality was the most concentrated (1,2x10³ JIs/cm²), which may suggest a positive correlation between dose and mortality. Contrary, B. bassiana Bb9205 and M. anisopliae Ma9236 showed a possible negative correlation, thus 1x10⁵ con/cm², the dose with de highest mortality, is one of the lowest doses tested. This result may be explained by Butt & Goettel (2000), who mentioned that for entomopathogenic fungi there is a threshold dose to kill a pest, nevertheless the exact nature of this relationship has not been defined yet. Studies by Goettel et al. (1993) and Vandenberg et al. (1998) report a negative correlation between doses and mortality at the highest concentrations, occurring an autoinhibition; which may explain our mortality results for the fungal strains.

This study is of high importance as it gives way to establish interactions between fungi and nematodes that can generate possible synergy or additivity to pest mortality.

Conclusion

The inoculations of entomopathogenic nematodes (1,2x10³ JIs/cm²) and fungi (1x10⁵ con/cm²) cause mortality rates exceeding 90% for P. xylostella, in comparison with the majority of studies using higher concentrations to achieve the same mortality. The use of Colombian strains of H. bacteriophora HNI0100, B. bassiana Bb9205 and M. anisopliae Ma9236 is an innovative alternative for the control of P. xylostella and should be considered a management strategy in broccoli production.

Acknowledgements

The authors wish to acknowledge the support of the Centro de Bio-Sistemas (C-BIOS) of University Jorge Tadeo Lozano and Pontificia Univerisidad Javeriana.

Conflicts of interest

The authors declare that there are no conflicts of interest regarding the results published in this work.

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