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Nova
Print version ISSN 1794-2470
Abstract
RODRIGUEZ PAVA, Cristian Nicolás; ZARATE SANABRIA, Andrés Gabriel and SANCHEZ LEAL, Ligia Consuelo. Antimicrobial activity of four varieties of plants against pathogens clinical significance in Colombia. Nova [online]. 2017, vol.15, n.27, pp.119-129. ISSN 1794-2470.
Method.
The methodology included the acquisition, drying, soaking, grinding, preparing ethanolic crude extracts and concentration by rotary evaporation; phytochemical analysis, visualization by thin layer chromatography. Antimicrobial tests were performed with different concentrations of extracts as indicated by the Clinical and Laboratory Standards Institute. The microorganisms used were Enterococcus faecium vancomycin-resistant, Streptococcus pneumoniae, Klebsiella pneumoniae with KPC, Providencia rettgeri presence of ESBLs, Pseudomonas aeruginosa, Enterobacter cloacae, Escherichia coli, Staphylococcus aureus (3-lysine and Candida albicans.
Results.
Chromatographies allowed checking the presence of flavonoids, terpenes, saponins, phenols, quinones and alkaloids have been reported having antimicrobial activity. In antimicrobial susceptibility tests it found that extracts showed varying degrees of inhibition against microorganisms study, the most effective stems T. officinale.
Conclusion.
It can be concluded that plant extracts may be alternative in the treatment of nosocomial infections.
Keywords : Bacterial resistance; medicinal plants; organic extracts; antimicrobial activity; susceptibility testing; phytochemical analysis.